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Comparison of Treponema pallidum genomes for the prediction of resistance genes

Author:
De Souza, Ronaldo Omizolo, da Silva, Kesia Esther, Pereira, Rodrigo Matheus, Simionatto, Simone
Source:
Journal of biosciences 2019 v.44 no.2 pp. 34
ISSN:
0250-5991
Subject:
Treponema pallidum, antibiotic resistance, antibiotic resistance genes, antibiotics, bioinformatics, drugs, genetic databases, microorganisms, molecular biology, prediction, prokaryotic cells, sexually transmitted diseases, Brazil
Abstract:
Syphilis is a sexually transmitted infection caused by Treponema pallidum, which is highly prevalent in several countries, including Brazil. The use of bioinformatics’ tools for the identification of resistance genes is an important practice for the study of microorganisms, such as T. pallidum. In this study, the complete genomes of 43 strains of T. pallidum, isolated from different countries, were analyzed. A total of 41,514 sequences were obtained, and compared against prokaryote resistance gene databases using BLASTn, BLASTx and RGI for gene alignment and prediction. From the alignments, it was possible to identify antibiotic resistance genes for each strain. The genes identified in each comparison were grouped according to the antibiotic category in which they show resistance to. The antibiotic-resistant genes related to drugs used to treat syphilis were grouped separately. The in silico tools used have shown to be effective in identifying resistance genes in genomes of T. pallidum strains. Due to the lack of research and accurate information regarding the antibiotic resistance genes in T. pallidum, this study serves as a basis for studies in molecular biology whose aim is the identification of these genes, besides being a reference to help in the control and treatment of this infection.
Agid:
6365127