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pH-Operated Triplex DNA Device on MoS₂ Nanosheets
- Ji, Wei, Li, Dan, Lai, Wei, Yao, Xiaowei, Alam, Md. Fazle, Zhang, Weijia, Pei, Hao, Li, Li, Chandrasekaran, Arun Richard
- Langmuir 2019 v.35 no.14 pp. 5050-5053
- DNA, biosensors, dyes, molybdenum disulfide, nanosheets, pH
- We report a triplex-based DNA device coupled with molybdenum disulfide (MoS₂) nanosheets for use as a pH-sensing platform. The device transitions from a duplex state at pH 8 to a triplex state at pH 5. The interaction of the device with MoS₂ nanosheets in the two states is read out as a fluorescence signal from a pH-insensitive dye attached to the device. We characterized the operation of the DNA device on MoS₂ nanosheets, analyzed the pH response, and tested the reversibility of the system. Our strategy can lead to the creation of a suite of biosensors where the sensing element is a triplex DNA device and the signal response is modulated by inorganic nanomaterials.