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Efficient gene disruption by posttransformational directed internal homologous recombination in Pichia pastoris
- Jiao, Liangcheng, Zhou, Qinghua, Yan, Yunjun
- Analytical biochemistry 2019 v.576 pp. 1-4
- Pichia pastoris, gene deletion, gene targeting, genes, homologous recombination, yeasts
- In this study, we designed an improved method to construct gene-deficient strain in non-conventional yeast Pichia pastoris. This method achieved high efficiency of gene deletion without disruption of NHEJ-related genes. The disruption efficiency of Och1 reached about 80%. This simple method will be a new useful molecular tool for P. pastoris.