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Antibodies against H1N1 influenza virus hemagglutinin cross-react with prohibitin

Sun, Lijun, Li, Huijin, Sun, Jingying, Guo, Chunyan, Feng, Yangmeng, Li, Yan, Zhao, Xiangrong, Xie, Xin, Hu, Jun
Biochemical and biophysical research communications 2019 v.513 no.2 pp. 446-451
Orthomyxoviridae, Western blotting, antigens, bacteria, cross reaction, hemagglutinins, humans, insulin-dependent diabetes mellitus, islets of Langerhans, mass spectrometry, monoclonal antibodies, pathogenesis, rats, transfection
Influenza virus infection is associated with type 1 diabetes (T1DM), but its pathogenesis remains unclear. Here, our study found that one of the monoclonal antibodies against H1N1 influenza virus hemagglutinin(HA) cross-reacted with human pancreatic tissue and further demonstrated that it binded to rat islet β-cells. We immunoprecipitated islet protein with this cross-reactive antibody and identified the bound antigen as prohibitin by mass spectrometry. We then expressed the prohibitin protein in bacteria and confirmed the antibody binding to prohibitin by Western blot. We also verified the cross-reactivity of the antibody by prohibitin-siRNA transfection in islet beta cells. We conclude that prohibitin is an autoantigen that cross-reacts with influenza virus HA. The correlation between the autoantigen prohibitin and type 1 diabetes remains to be investigated.