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Human mastadenovirus in water, sediment, sea surface microlayer, and bivalve mollusk from southern Brazilian beaches

Gularte, Juliana Schons, Girardi, Viviane, Demoliner, Meriane, de Souza, Fernanda Gil, Filippi, Micheli, Eisen, Ana Karolina Antunes, Mena, Kristina D., de Quevedo, Daniela Muller, Rigotto, Caroline, de Barros, Marcelo Pereira, Spilki, Fernando Rosado
Marine pollution bulletin 2019 v.142 pp. 335-349
Bivalvia, Escherichia coli, Hepatitis E virus, Mastadenovirus, air, beaches, genomics, microbiological risk assessment, quantitative polymerase chain reaction, risk, sediments, water pollution, Brazil
Anthropogenic contamination of beaches in the south of Brazil was assessed by detection of Escherichia coli, human mastadenovirus species C (HAdV-C) and F (HAdV-F) and hepatitis E virus (HEV). Sampling was carried out in October (2016), and in January, April and July (2017). Water, sediment, sea surface microlayer (SML), bivalves, and air sentinel samples were evaluated. Quantitative microbiological risk assessment (QMRA) was used to estimate the probability of swimmer infection. HAdV-C was present in 26% of the samples, for both qPCR and viral isolation. The highest rates of detection in genomic copies (GC) were in water (2.42E+10 GC/L), SML (2.08E+10 GC/L), sediment (3.82E+08 GC/g) and bivalves (3.91E+07 GC/g). QMRA estimated daily and annual risks with a maximum value (9.99E-01) in almost all of the samples. Viable HAdV-C was often detected in the SML, pointing that this is a source of infection for people bathing in these waters.