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Construction of a recombinant BHV-1 expressing the VP1 gene of foot and mouth disease virus and its immunogenicity in a rabbit model

Ren, Xian-Gang, Xue, Fei, Zhu, Yuan-Mao, Tong, Guang-Zhi, Wang, Yan-Hui, Feng, Jun-Ke, Shi, Hong-Fei, Gao, Yu-Ran
Biotechnology letters 2009 v.31 no.8 pp. 1159-1165
Human herpesvirus 5, enzyme-linked immunosorbent assay, foot-and-mouth disease, genes, homologous recombination, immune response, infectious bovine rhinotracheitis, loci, models, neutralization tests, rabbits, somatotropin, vaccines, viruses
Foot-and-mouth disease (FMD) and infectious bovine rhinotracheitis (IBR) are two important infectious diseases of cattle. Using bovine herpesvirus type 1 (BHV-1) as a gene delivery vector for development of live-viral vaccines has gained widespread interest. In this study, a recombinant BHV-1 was constructed by inserting the synthetic FMDV (O/China/99) VP1 gene in the the gE locus of BHV-1 genome under the control of immediately early gene promoter of human cytomegalovirus (phIE CMV) and bovine growth hormone polyadenylation (BGH polyA) signal. After homologous recombination and plaque purification, a recombinant virus named BHV-1/gE⁻/VP1 was acquired and identified. The immunogenicity was confirmed in a rabbit model by virus neutralization test and enzyme-linked immunosorbent assay (ELISA). The result indicated that the BHV-1/gE⁻/VP1 has the potential for being developed as a bivalent vaccine for FMD and IBR.