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Host bacterial community of MGEs determines the risk of horizontal gene transfer during composting of different animal manures

Zhu, Longji, Zhao, Yue, Yang, Kangjie, Chen, Jian, Zhou, Haixuan, Chen, Xiaomeng, Liu, Qi, Wei, Zimin
Environmental pollution 2019 v.250 pp. 166-174
ammonium, antibiotic resistance genes, bacteria, bacterial communities, cattle manure, composting, interspersed repetitive sequences, nitrates, pH, poultry manure, risk
Mobile genetic elements (MGEs) play critical roles in transferring antibiotic resistance genes (ARGs) among different microorganisms in the environment. This study aimed to explore the fate of MGEs during chicken manure (CM) and bovine manure (BM) composting to assess horizontal transfer risks of ARGs. The results showed that the removal efficiency of MGEs during CM composting was significantly higher than that during BM composting, because the potential host bacteria of MGEs were eliminated largely during CM composting. Meanwhile, these potential host bacterial communities are significantly influenced by pH, NH4+, NO3− and total N, which can be used to regulate host bacterial communities to remove MGEs during composting. Projection pursuit regression further confirmed that composting can effectively reduce the horizontal transfer risk of ARGs, especially for CM composting. These results identified the critical roles of host bacterial communities in MGEs removal during composting of different animal manures.