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Optimization of light intensity and photoperiod for Isochrysis galbana culture to improve the biomass and lipid production using 14-L photobioreactors with mixed light emitting diodes (LEDs) wavelength under two-phase culture system
- Che, Clovis Awah, Kim, So Hee, Hong, Hee Jun, Kityo, Moses Katongole, Sunwoo, In Yung, Jeong, Gwi-Taek, Kim, Sung-Koo
- Bioresource technology 2019 v.285 pp. 121323
- Isochrysis galbana, biomass production, light emitting diodes, light intensity, lipid content, photobioreactors, stearic acid, wavelengths
- The optimal light intensity and photoperiod required to produce high biomass and lipid contents in Isochrysis galbana cultured in a 14-L bioreactor with LED wavelengths was studied. The cell biomass production was monitored in the first phase comprising of mixed blue (465 nm) and red (640 nm) LED wavelengths, then green (520 nm) LED were used in the second phase for lipid production. The optimal light intensity was 400 µmol/m2/s giving a maximum cell biomass of 1.05 g dcw/L and total lipid content of 65.2% (w/w) cultured under 12:12 h L/D cycle. The optimal light intensity of 400 µmol/m2/s was applied at different L/D cycles, the maximum cell biomass (1.25 g dcw/L) and lipid content (71.1% w/w) were obtained at 18:6 h L/D cycle. Stearic acid was the main fatty acid ranging from 42.91 (500 µmol/m2/s) to 65.57% w/w (100 µmol/m2/s) and 53.84 (18:6 h) to 65.44% w/w (24:0 h).