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Prediction of ligand modulation patterns on membrane receptors via lysine reactivity profiling

Author:
Zhou, Ye, Liu, Zheyi, Zhang, Jinbao, Dou, Tongyi, Chen, Jin, Ge, Guangbo, Zhu, Shujia, Wang, Fangjun
Source:
Chemical communications 2019 v.55 no.30 pp. 4311-4314
ISSN:
1364-548X
Subject:
catechol O-methyltransferase, chemical reactions, ligands, lysine, mass spectrometry, prediction, receptors
Abstract:
Herein we provide a mass spectrometry-based lysine reactivity profiling strategy to monitor the ligand modulation of protein receptors, which is achieved by active dimethyl labeling of lysine residues and comparison of the alterations of labeling reactivity during ligand binding. The small-molecule ligand modulation patterns on the catechol-O-methyltransferase and the N-methyl-d-aspartate receptors have been predicted, including both binding regions and related conformational changes.
Agid:
6368121