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Development of a high sensitivity quantum dot-based fluorescent quenching lateral flow assay for the detection of zearalenone
- Chen, Yuan, Fu, Qiangqiang, Xie, Jun, Wang, Hong, Tang, Yong
- Analytical and bioanalytical chemistry 2019 v.411 no.10 pp. 2169-2175
- carcinogenicity, corn, detection limit, fluorescence, quantum dots, screening, zearalenone
- Zearalenone (ZEN) is a common carcinogenic toxin related to cereal contamination. In this study, we developed a high sensitivity quantum dot (QD)-based fluorescent quenching lateral flow assay (LFA) for sensitive ZEN detection. The linear detection range of the fluorescent quenching LFA for ZEN was 0.78–25 ng/mL, and the limit of detection was 0.58 ng/mL. In addition, the fluorescent quenching LFA showed high recovery (83.1–93.6%) for detection of ZEN concentrations spiked into corn samples. These results indicate that the QD-based fluorescent quenching LFA may be a valuable tool for preliminary screening of ZEN contamination.