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NMR for metabolomics studies of Crataegus rhipidophylla Gand.
- Kumar, Dinesh, Thakur, Krishana, Sharma, Shruti, Kumar, Shiv
- Analytical and bioanalytical chemistry 2019 v.411 no.10 pp. 2149-2159
- Crataegus, aerial parts, amygdalin, carbon, chemical composition, epicatechin, flowers, fruits, leaves, liver, liver diseases, metabolites, metabolomics, neoplasms, nuclear magnetic resonance spectroscopy, plant response, plant tissues, quality control, quantum mechanics, rutin, seeds, shikimic acid, stable isotopes, stems, world markets
- Crataegus products for food, nutritional, and pharmaceutical uses are available over the counter in the global market. The aerial parts are enriched with biochemicals and are used to treat cardiovascular, respiratory, and liver disorders and cancer. Detailed study to understand the chemical composition and different levels within medicinally important plant tissues needs hours, such as plant response for metabolite flux and quality control purposes. Therefore, comprehensive metabolite profiling (qualitatively and quantitatively) of different tissues [leaves, flowers, stems, fruits (without seeds), and seeds; n = 5] of Crataegus rhipidophylla Gand. was performed by NMR techniques in one session. Fifty-eight compounds of different classes were unambiguously identified with the use of 1D and 2D NMR techniques (¹H, ¹³C, distortionless enhancement by polarization transfer with 135° angle, correlation spectroscopy, heteronuclear single quantum coherence, heteronuclear multiple bond correlation) and quantified by a relative quantification method. Chemical compounds such as amygdalin (seeds), rutin (flowers), isovitexin (fruits), shikimic acid (leaves), and epicatechin (stems) were identified as authentication as well as discrimination markers for the respective C. rhipidophylla tissues. The fruits contain a significant mixture of important metabolites, followed by flowers and leaves. Trends of metabolite levels, upregulation, and downregulation in plant tissues were found, which highlights the responses of plant tissues in a specific time. NMR-based comprehensive metabolite information was used to develop the chemical signature, quality control chemical markers, and strategies to understand the chemical diversity of C. rhipidophylla. This chemical information from different aerial tissues will provide guidance for the extensive utilization of Crataegus.