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Fluorometric Determination of Artemisinin Using the Pyronin B–Microperoxidase-11 System

Muginova, S. V., Vakhraneva, E. S., Myasnikova, D. A., Shekhovtsova, T. N.
Journal of analytical chemistry 2019 v.74 no.2 pp. 100-107
Artemisia annua, artemisinin, biomimetics, cytochrome c, dietary supplements, dyes, fluorescence, fluorometry, hemoglobin, oligopeptides, peroxidase, spectroscopy, xanthenes
A sensitive, rapid, and simple fluorimetric procedure for the determination of artemisinin in a concentration range of 0.1–7 μM was developed with the use of microperoxidase-11 as a peroxidase biomimetic (RSD = 0.8% at LOQ, n = 5; LOD = 7.1 nM (3s₀)). The determination is based on the fluorescence quenching of the cationic xanthene dye pyronin B (Stern–Volmer quenching constant, 0.101 μM–¹) in the presence of microperoxidase-11. The procedure was tested in the analysis of a biologically active additive based on an Artemisia annua wormwood extract. The correctness of the results of the fluorimetric determination of artemisinin in a biologically active dietary supplement was confirmed by HPLC–mass spectrometry. The use of oligopeptide microperoxidase-11 instead of heme-containing proteins (hemoglobin, cytochrome c, and horseradish peroxidase) made it possible to shorten the duration of artemisinin determination by a factor of 2 with the retention of sensitivity and selectivity.