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Sydnone-based turn-on fluorogenic probes for no-wash protein labeling and in-cell imaging
- Plougastel, Lucie, Pattanayak, Manas R., Riomet, Margaux, Bregant, Sarah, Sallustrau, Antoine, Nothisen, Marc, Wagner, Alain, Audisio, Davide, Taran, Frédéric
- Chemical communications 2019 v.55 no.31 pp. 4582-4585
- chemical compounds, cycloaddition reactions, image analysis
- We report the synthesis and use of sydnone-based profluorophores as tools for imaging applications. These new probes display exquisite reactivity towards strain promoted cycloaddition reactions with cycloalkynes allowing fast, efficient and selective labeling in biological media. Styryl-pyridinium sydnone probes were found particularly interesting for click reactions to proceed selectively inside cells.