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Effects of cerium oxide nanoparticles on bacterial growth and behaviors: induction of biofilm formation and stress response

Xu, Yi, Wang, Chao, Hou, Jun, Wang, Peifang, You, Guoxiang, Miao, Lingzhan
Environmental science and pollution research international 2019 v.26 no.9 pp. 9293-9304
Citrobacter, Pseudomonas, adenosine triphosphate, bacteria, bacterial growth, biofilm, biomass, ceric oxide, environmental impact, homoserine, hydrophobicity, microstructure, nanoparticles, oxidative stress, polysaccharides, quorum sensing, stress response
In this paper, the effects of cerium oxide nanoparticles (CeO₂ NPs) on the group bacterial behaviors were elaborated. After 36-h cultivation, the biofilm biomass was enhanced by the sub-lethal concentrations of 0.5 and 2 mg/L CeO₂ NP exposure. Meanwhile, the promoted production of total amino acids in microbes further resulted in the increased surface hydrophobicity and percentage aggregation. To resist the CeO₂ NPs stress, the biofilm exhibited a double-layer microstructure, with the protein (PRO) and living cells occupying the bottom, the polysaccharide (PS), and dead cells dominating the top. The bacterial diversity was highly suppressed and Citrobacter and Pseudomonas from the phylum of γ-Proteobacteria strongly dominated the biofilm, indicating the selective and enriched effects of CeO₂ NPs on resistant bacteria. The stimulated inherent resistance of biofilm was reflected by the reduced adenosine triphosphate (ATP) content after 4 h exposure. The increased levels of reactive oxygen species (ROS) in the treatments of 8 h CeO₂ NP exposure led to the upregulated quorum sensing signals of acylated homoserine lactone (AHL) and autoinducer 2 (AI-2), beneficial to mitigating the environmental disturbance of CeO₂ NPs. These results provide evidences for the accelerating effects of CeO₂ NPs on biofilm formation through oxidative stress, which expand the understanding of the ecological effects of CeO₂ NPs.