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Inhibitory effects of punicalagin from Punica granatum against type II collagenase-induced osteoarthritis

Lee, Chia-Jung, Chen, Lih-Geeng, Liang, Wen-Li, Hsieh, Ming-Shium, Wang, Ching-Chiung
Journal of functional foods 2018 v.41 pp. 216-222
Punica granatum, acetone, active ingredients, animal models, chondrocytes, functional foods, inducible nitric oxide synthase, inflammation, inhibitory concentration 50, interleukins, laboratory animals, matrix metalloproteinase 13, nociception, osteoarthritis, pomegranates, prostaglandin synthase, prostaglandins, protein synthesis, rats
Pomegranate (Punica granatum) is a heath with abundant hydrolysable tannin in its peel. This study demonstrated that POMx (70% acetone extract of pomegranate peels) significantly reduced interleukin (IL)-1β-induced inducible nitric oxide synthase, cyclooxygenase-2, and matrix metalloproteinase-13 protein expression in primary rat chondrocytes (PRCs). In the type II collagenase-induced osteoarthritis rat model, POMx (150 mg/kg) recovered the weight-bearing ratio changes in experimental animals, suggesting the alleviation of knee-related inflammation and nociception. Therefore, the major compound, punicalagin, was isolated from POMx. Punicalagin also significantly reduced IL-1β-induced inflammatory factors in PRCs and exerted significant antiosteoarthritis effects in the vivo model after 28-d treatment (concentration: 0.50 mg/kg). However, the IC50 values of POMx and punicalagin against prostaglandin E2 production were 83.2 and 36.0 μg/mL, respectively, and the concentration of punicalagin in POMx was 19.1%. Altogether, POMx can be used to develop functional foods for knee-related diseases, and punicalagin can act as an active ingredient.