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Expression of NAD⁺-dependent formate dehydrogenase in Enterobacter aerogenes and its involvement in anaerobic metabolism and H₂ production
- Lu, Yuan, Zhao, Hongxin, Zhang, Chong, Lai, Qiheng, Wu, Xi, Xing, Xin-Hui
- Biotechnology letters 2009 v.31 no.10 pp. 1525-1530
- Candida boidinii, Enterobacter aerogenes, NAD (coenzyme), anaerobiosis, hydrogen
- An expression system for NAD⁺-dependent formate dehydrogenase gene (fdh1), from Candida boidinii, was constructed and cloned into Enterobacter aerogenes IAM1183. With the fdh1 expression, the total H₂ yield was attributed to a decrease in activity of the lactate pathway and an increase of the formate pathway flux due to the NADH regeneration. Analysis of the redox state balance and ethanol-to-acetate ratio in the fdhl-expressed strain showed that increased reducing power arose from the reconstruction of NADH regeneration pathway from formate thereby contributing to the improved H₂ production.