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Transaminase-catalyzed asymmetric synthesis of l-2-aminobutyric acid from achiral reactants
- Shin, Jong-Shik, Kim, Byung-Gee
- Biotechnology letters 2009 v.31 no.10 pp. 1595-1599
- Vibrio fluvialis, benzaldehyde, hexane
- Asymmetric synthesis of an unnatural amino acid was demonstrated by ω-transaminase from Vibrio fluvialis JS17. l-2-Aminobutyric acid was synthesized from 2-oxobutyric acid and benzylamine with an enantiomeric excess higher than 99%. The reaction showed severe product inhibition by benzaldehyde, which was overcome by employing a biphasic reaction system to remove the inhibitory product from the aqueous phase. In a typical biphasic reaction (50 mM 2-oxobutyric acid, 70 mM benzylamine and 2.64 U/ml purified enzyme) using hexane as an extractant, conversion of 2-oxobutyric acid reached 96% in 5 h whereas only 39% conversion was obtained without the product extraction.