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Discriminative Detection of Glutathione in Cell Lysates Based on Oxidase-Like Activity of Magnetic Nanoporous Graphene

Author:
Zhang, Haijuan, Chen, Jia, Yang, Yali, Wang, Li, Li, Zhan, Qiu, Hongdeng
Source:
Analytical chemistry 2019 v.91 no.8 pp. 5004-5010
ISSN:
1520-6882
Subject:
combustion, detection limit, electron transfer, enzymes, ferric chloride, fluorescence, glutathione, graphene, graphene oxide, magnetism, nanocomposites, nanoparticles, nanopores, physiological transport, surface area, thiamin
Abstract:
As the most abundant intracellular biothiol, glutathione (GSH) plays a central role in many cellular functions and has been proved to be associated with numerous clinical diseases. Nevertheless, it is still a challenge to detect GSH over other mercaptoamino acids owing to their similar structures and activities. In this paper, magnetic nanoporous graphene (MNPG) nanocomposites were prepared for the first time through partial combustion of graphene oxide (GO) and ferric chloride. Due to the combination of porous graphene and magnetic nanoparticles, the MNPG nanocomposites exhibited large specific surface area, fast mass, and electron transport kinetics, resulting in remarkable oxidase mimic activity and easy separation. On the basis of the inhibition effect of GSH on the MNPG-catalyzed oxidation of thiamine, a novel and simple method for fluorescence determination of GSH was established. The sensor displayed a good linear response in the range of 0.2–20 μM toward GSH with a limit of detection of 0.05 μM. High sensitivity and selectivity facilitated its practical application for discriminative detection of GSH levels in PC12 cell lysates. The presented assay will be a simple and powerful tool to monitor intracellular GSH levels for biomedical diagnosis. Furthermore, the MNPG nanocomposites will provide insights to construct nanoporous graphene-based hybrids and push forward the advancement of porous graphene for wide applications.
Agid:
6371875