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Testosterone improves the osteogenic potential of a composite in vitro and in vivo

da Costa, Kelen J. R., Gala-García, Alfonso, Passos, Joel J., Santos, Vagner R., Sinisterra, Ruben D., Lanza, Célia R. M., Cortés, Maria E.
Cell and tissue research 2019 v.376 no.2 pp. 221-231
adsorption, alkaline phosphatase, angiogenesis, bone formation, calcium phosphates, collagen, energy-dispersive X-ray analysis, enzyme activity, mineralization, nitric oxide, osteoblasts, osteocalcin, polymers, scanning electron microscopy, testosterone, thermal analysis, viability
Testosterone (T) has been suggested as a promising agent in the bone osteointegration when incorporated in a bioceramic/polymer combination for the local application. The objective of this study was to evaluate the activity of a testosterone composite of poly (lactic-co-glycolic acid) (PLGA), polycaprolactone (PCL), and biphasic calcium phosphate (BCP) as a strategy for enhancing its osteogenic effect and to evaluate tissue response to the composite implantation. PLGA/PCL/BCP/T and PLGA/PCL/BCP composites were prepared and characterized using thermal analysis. Composite morphology and surface characteristics were assessed by SEM and EDS. The evaluations of in vitro effects of testosterone composite on osteoblasts viability, alkaline phosphatase activity, collagen production, osteocalcin concentration, quantification of mineralization, and nitric oxide concentration, after 7, 14, and 21 days. Testosterone was successfully incorporated and composites showed a homogeneously distributed porous structure. The PLGA/PCL/BCP/T composite had a stimulatory effect on osteoblastic activity on the parameters evaluated, except to nitric oxide production. After 60 days, the PLGA/PCL/BCP/T composite showed no chronic inflammatory infiltrate, whereas the PLGA/PCL/BCP composite showed mild chronic inflammatory infiltrate. Angiogenesis, cellular adsorption, and fibrous deposit were observed on the surfaces of implanted composites. The composites in combination with testosterone can be exploited to investigate the use of this scaffold for bone integration.