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Dietary selenium supplementation alleviates immune toxicity in the hearts of chickens with lead-added drinking water

Author:
Huang, He, Jiao, Xiaoyan, Xu, Yanmin, Han, Qi, Jiao, Wanying, Liu, Yanyan, Li, Shu, Teng, Xiaohua
Source:
Avian pathology 2019 v.48 no.3 pp. 230-237
ISSN:
1465-3338
Subject:
chickens, dietary supplements, drinking water, gene expression, heart, heat shock proteins, inducible nitric oxide synthase, interferon-gamma, interleukin-17, interleukin-2, interleukin-4, interleukin-6, lead, lead acetate, lead poisoning, males, messenger RNA, nitric oxide, pollutants, protein synthesis, quantitative polymerase chain reaction, selenium, sodium selenite, toxicity
Abstract:
Lead (Pb) is an environmental pollutant and can damage organisms. Selenium (Se) can alleviate Pb poisoning. The present study aimed to investigate the alleviative effect of Se on Pb-induced immune toxicity in chicken hearts. One-hundred-and-eighty Hy-line male chickens were randomly divided into four groups at 7 days of age. The control group was offered a standard commercial diet (SD) and drinking water (DW); the Se group was offered SD supplemented with sodium selenite (SeSD) and DW; the Pb + Se group was offered SeSD and DW supplemented with lead acetate (PbDW); and the Pb group was offered SD and PbDW. Relative mRNA expression of inducible nitric oxide synthase (iNOS), interleukins (IL-2, IL-4, IL-6, IL-12β, IL-17 and IFN-γ), and heat shock proteins (HSP27, HSP40, HSP60, HSP70, and HSP90) were determined by means of quantitative real-time PCR. Relative protein expression of iNOS, HSP60, HSP70, and HSP90 was assessed, as well as nitric oxide (NO) content and iNOS activity in heart tissue. The results indicated a down-regulation of interleukin (IL)-2 and IFN-γ and an up-regulation of NO, iNOS, interleukins (IL-4, IL-6, IL-12β, IL-17), and heat shock proteins (HSP27, HSP40, HSP60, HSP70, and HSP90) in Pb-damaged hearts. Se alleviated all of the above Pb-induced changes. There were time-dependent effects on NO content, iNOS activity, and mRNA levels of iNOS, IL-2, IL-4, IL-6, IL-17, HSP27, HSP40, HSP60, HSP70, and HSP90 after Pb treatment in the chicken hearts. Se alleviated Pb-induced immune toxicity in the chicken hearts.
Agid:
6372537