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Identification of Differentially Expressed Genes of Rice Under Cadmium Stress Using DDRT-PCR Approach
- Wang, Jian-hui, Zhao, Hai-Ming, Mo, Ce-hui, Hou, Xue-wen
- Bulletin of environmental contamination and toxicology 2019 v.102 no.4 pp. 589-594
- adenosinetriphosphatase, aquaporins, cadmium, cultivars, drug resistance, food plants, genes, human health, nitrates, nutrient solutions, nutrient uptake, plasma membrane, pollutants, protein kinases, reverse transcriptase polymerase chain reaction, rice, root tips, seedlings
- Cadmium (Cd) is one of the hazardous environmental pollutants, and it can be harmful to human health through consumption of food-plants capable of bioaccumulating Cd. Therefore, lowering cadmium accumulation in plants is highly desirable. Here, a rice cultivar ‘Qisanzhan’ was studied using differential display reverse transcription-polymerase chain reaction (DDRT-PCR). Fifty-six differentially expressed genes were found in the root tips of 4-leaf stage rice seedlings exposed to 4 and 12 h of 50 µmol/L Cd(NO₃)₂ in a nutrient solution using DDRT-PCR. Further validation using semi-quantitative RT-PCR showed that the expression patterns of 16 genes were consistent with those found in DDRT-PCR. These genes encode receptor-like protein kinase, pleiotropic drug resistance protein, aquaporin protein, plasma membrane ATPase, etc. The differentially genes identified here can be used to obtain a better understanding of the molecular mechanisms of Cd absorption and accumulation in plants.