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Identification of Differentially Expressed Genes of Rice Under Cadmium Stress Using DDRT-PCR Approach

Wang, Jian-hui, Zhao, Hai-Ming, Mo, Ce-hui, Hou, Xue-wen
Bulletin of environmental contamination and toxicology 2019 v.102 no.4 pp. 589-594
adenosinetriphosphatase, aquaporins, cadmium, cultivars, drug resistance, food plants, genes, human health, nitrates, nutrient solutions, nutrient uptake, plasma membrane, pollutants, protein kinases, reverse transcriptase polymerase chain reaction, rice, root tips, seedlings
Cadmium (Cd) is one of the hazardous environmental pollutants, and it can be harmful to human health through consumption of food-plants capable of bioaccumulating Cd. Therefore, lowering cadmium accumulation in plants is highly desirable. Here, a rice cultivar ‘Qisanzhan’ was studied using differential display reverse transcription-polymerase chain reaction (DDRT-PCR). Fifty-six differentially expressed genes were found in the root tips of 4-leaf stage rice seedlings exposed to 4 and 12 h of 50 µmol/L Cd(NO₃)₂ in a nutrient solution using DDRT-PCR. Further validation using semi-quantitative RT-PCR showed that the expression patterns of 16 genes were consistent with those found in DDRT-PCR. These genes encode receptor-like protein kinase, pleiotropic drug resistance protein, aquaporin protein, plasma membrane ATPase, etc. The differentially genes identified here can be used to obtain a better understanding of the molecular mechanisms of Cd absorption and accumulation in plants.