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Reversible “turn-off-on” fluorescence response of Fe(III) towards Rhodamine B based probe in vivo and plant tissues
- Song, Fan, Shao, Xiaotao, Zhu, Jing, Bao, Xiaofeng, Du, Lei, Kan, Chun
- Tetrahedron letters 2019 v.60 no.20 pp. 1363-1369
- animals, chemical reactions, chemical structure, fluorescence, iron, monitoring, plant tissues, rhodamines, stoichiometry
- A Fe3+-specific probe (N-TC) based on Rhodamine B was designed and synthesized. N-TC has a good spectral response to Fe3+ in the EtOH/H2O solution (1:1, v/v, HEPES, 0.5 mM, pH = 7.38) with low detection limits and high binding constants. N-TC displays the reversible “turn-off-on” fluorescence response with 1:1 binding stoichiometry. It is further proven to be practical in sensitively monitoring trace Fe3+ in environmental water specimens. Biological experiments demonstrated that N-TC can be respectively used as a probe for detection of Fe3+ in living cells, animals and plant tissues.