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BRB-seq: ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing
- Alpern, Daniel, Gardeux, Vincent, Russeil, Julie, Mangeat, Bastien, Meireles-Filho, Antonio C. A., Breysse, Romane, Hacker, David, Deplancke, Bart
- Genome biology 2019 v.20 no.1 pp. 71
- DNA barcoding, RNA, cDNA libraries, complementary DNA, gene expression, genes, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, transcriptomics
- Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time. BRB-seq has a comparable performance to the standard TruSeq approach while showing greater tolerance for lower RNA quality and being up to 25 times cheaper. We anticipate that BRB-seq will transform basic laboratory practice given its capacity to generate genome-wide transcriptomic data at a similar cost as profiling four genes using RT-qPCR.