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Cadmium-induced oxidative stress in Prussian carp (Carassius gibelio Bloch) hepatopancreas: ameliorating effect of melatonin

Author:
Drąg-Kozak, Ewa, Pawlica-Gosiewska, Dorota, Gawlik, Katarzyna, Socha, Magdalena, Gosiewski, Grzegorz, Łuszczek-Trojnar, Ewa, Solnica, Bogdan, Popek, Włodzimierz
Source:
Environmental science and pollution research international 2019 v.26 no.12 pp. 12264-12279
ISSN:
0944-1344
Subject:
Carassius gibelio, antioxidants, cadmium, copper, enzyme activity, exposure duration, females, fish, glutathione, glutathione peroxidase, glutathione-disulfide reductase, hepatopancreas, iron, melatonin, oxidative stress, superoxide dismutase, tissues, toxicity, zinc
Abstract:
The oxidative status of the hepatopancreas of Prussian carp females (Carassius gibelio) co-exposed to sublethal cadmium in water and melatonin was studied. The activities of antioxidant enzymes such as glutathione reductase (GR), glutathione peroxidase (GPx), and superoxide dismutase (SOD) as well as the concentration of reduced glutathione (GSH) were measured in homogenates of the hepatopancreas. Furthermore, concentrations of cadmium (Cd), zinc (Zn), copper (Cu), and iron (Fe) in the hepatopancreas were assayed. These females received melatonin implants and were exposed to 0.4 mg/L or 4.0 mg/L Cd in water for either a 13- or a 7-week period, followed by further 6 weeks of purification in clear water. Exposure to Cd influenced the increase in this metal concentration in fish hepatopancreas. In contrast, the fish exposed to cadmium with additional administration of melatonin had a lower accumulation of this metal. Exposure to Cd caused the increase in GSH content and the activity of GR, and a reduction in GPx activity, whereas the SOD activity varies depending on the exposure time on cadmium. In the hepatopancreas of fish treated with Cd alone, the content of Cu and Zn were increased and that of Fe was changed. After melatonin administration to Cd-exposed fish, a decrease in copper and zinc hepatopancreas content was noted. The present findings imply that melatonin co-treatment can effectively protect the fish against the toxic effects of cadmium on endogenous antioxidant status in hepatopancreas tissues and variations in metal concentration, such as Zn, Cu, and Fe.
Agid:
6377771