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Determination of the 5′-terminal sequence of subgenomic RNA of hepatitis E virus strains in cultured cells
- Ichiyama, Koji, Yamada, Kentaro, Tanaka, Toshinori, Nagashima, Shigeo, Jirintai, Takahashi, Masaharu, Okamoto, Hiroaki
- Archives of virology 2009 v.154 no.12 pp. 1945-1951
- Orthohepevirus A, complementary DNA, mutation, clones, cultured cells, genotype, RNA
- Using RNA preparations extracted from PLC/PRF/5 cells transfected with infectious genotype 3 hepatitis E virus (HEV) cDNA clones or inoculated with a fecal suspension containing a genotype 4 HEV, the 5′-terminal sequence of a 2.2-kb subgenomic RNA of genotype 3 and 4 HEVs was determined. Despite an insertion of T after nucleotide 5116 or an ORF3-null mutation in genotype 4 HEV and/or one of the genotype 3 variants, it was found that the subgenomic RNA of genotype 3 and 4 HEVs initiates exclusively at nucleotide 5122 with the common sequence of 5′-GC, which is identical to that of the prototype genotype 1 HEV.