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High uptake carboxyl-functionalized porous β-cyclodextrin polymer for selective extraction of lysozyme from egg white

Author:
Duan, Hui-Ling, Niu, Qi-Le, Wang, Jun, Ma, Shi-Yao, Zhang, Jing, Zhang, Zhi-Qi
Source:
Journal of chromatography 2019 v.1600 pp. 80-86
ISSN:
0021-9673
Subject:
adsorbents, adsorption, beta-cyclodextrin, chromatography, egg albumen, hydrolysis, lysozyme, moieties, polymers
Abstract:
Lysozyme is widely used in medical, food and industrial fields due to its bacteriolytic effect and thus it is significant to design and develop a specific adsorbent with high adsorption capacity and selectivity for lysozyme. Inspired by the high uptake capacity of tetrafluoroterephthalonitrile-crosslinked porous β-cyclodextrin polymers (P-CDPs) and the noncovalent interaction between lysozyme and carboxyl groups, the carboxyl-functionalized P-CDPs (P-CDP-COO−) were synthesized by base-catalysed hydrolysis of nitrile group in P-CDPs to carboxyl. Porous structure, large extent of carboxylic functional groups, cyclodextrin's preventing aggregation, and negatively charged make P-CDP-COO− possess an outstanding adsorption capability for lysozyme. The maximum saturated adsorption capacity reaches 1520 mg g−1, which is much better than the parent polymer and other reported materials. The as-prepared material was successfully utilized for the selective extraction of lysozyme from egg white.
Agid:
6378694