PubAg

Main content area

Benzonitriles as tyrosinase inhibitors with hyperbolic inhibition manner

Author:
Nihei, Ken-ichi, Kubo, Isao
Source:
International journal of biological macromolecules 2019 v.133 pp. 929-932
ISSN:
0141-8130
Subject:
active sites, copper, enzyme inhibition, enzyme inhibitors, enzymes, inhibitory concentration 50, mushrooms, oxidation
Abstract:
As a novel mushroom tyrosinase inhibitor, 4-methoxybenzonitrile (anisnitrile) was identified (IC50 = 111.1 μM) with hyperbolic inhibition manner. The calculated αKi (166.3 μM) was larger than Ki (66.5 μM) by Dixon plots, indicating that this nitrile acts as a competitive-noncompetitive mixed type inhibitor. Similarly, 4-isopropylbenzonitrile (cuminnitrile) partially inhibited the oxidation catalyzed by tyrosinase (IC50 = 121.5 μM, Ki = 88.8 μM, and αKi = 239.8 μM). Nine other benzonitriles also exhibited partial tyrosinase-inhibitory activity. In particular, 4-methylbenzonitrile (IC50 = 79.9 μM) is considered to be the most potent among the tested benzonitriles. Benzonitriles barely caused intermolecular amidine formation under physiologic conditions. Furthermore, they possibly coordinate copper at the active site of tyrosinase. Hence, benzonitriles exhibit different inhibition characteristics as compared with that exhibited by benzaldehydes.
Agid:
6379289