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YTOX: a rapid toxicity test based on the dehydrogenase activity of Saccharomyces cerevisiae for detection of contaminants in water samples

Author:
Gomes, Luiz Humberto, Duarte, Keila Maria Roncato, Kamogawa, Marcos Yassuo, Ferrarezi, Jessica Aparecida, Andrino, Felipe Gabriel, Tavares, Ana Claudia LoBuono, da Silva, Ana Paula Maria, Laguna, Sergio Escheverrigaray, de Lira, Simone Possedente
Source:
Journal of microbiological methods 2019 v.161 pp. 43-46
ISSN:
0167-7012
Subject:
Saccharomyces cerevisiae, bakers yeast, cadmium, dimethyl sulfoxide, enzyme activity, fipronil, toxic substances, toxicity, toxicity testing, water pollution
Abstract:
A simple generic toxicity method (test) is proposed using baker's yeast to mediate the reduction of the colourless triphenyltetrazolium chloride (TTC) to red, 1,3,5-triphenyl formazan, which can be extracted by dimethyl sulfoxide (DMSO), enabling the identification of reducible toxic compounds (e.g. cadmium, fipronil) in water for consumption.
Agid:
6379452