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The identification and isolation of anti-inflammatory ingredients of ethno medicine Breynia fruticosa

He, Xiao-Li, Lv, Jun-Jiang, Wang, Xu, Zhang, Qing, Zhang, Bei, Cao, Kun, Liu, Lu-Lu, Xu, Yi
Journal of ethnopharmacology 2019 v.239 pp. 111894
Breynia, adjuvants, animal models, anti-inflammatory activity, arthritis, eczema, gastroenteritis, high performance liquid chromatography, indomethacin, ingredients, mass spectrometry, nuclear magnetic resonance spectroscopy, permeability, pharyngitis, rats, resins, sesquiterpenoids, sulfur, tannins, toxicity, traditional medicine, China
Breynia fruticosa is a folk medicine in China, traditionally used to treat gastroenteritis, sore throat, eczema and arthritis. However, the bioactive ingredients are unknown.To identify and isolate the anti-inflammatory ingredients of B. fruticosa.B. fruticosa extracts were fractioned by Amberchrom CG161M and Toyopearl HW40C resins. Acetic acid-induced capillary permeability mice model was used to evaluate the anti-inflammation activities of fractions. The anti-inflammatory ingredients were identified by high performance liquid chromatography/mass spectroscopy (HPLC-MS). On-line two dimensional liquid chromatography system was constructed to remove the tannins and enrich the breynins. The breynins were purified by preparative HPLC and evaluated for their anti-arthritis activities using complete Freund's adjuvant (CFA) induced arthritis rats model.The anti-inflammatory ingredients of B. fruticosa are sulfur containing sesquiterpenoids (breynins). The on-line two dimensional preparative liquid chromatography system can effectively remove the tannins and enrich the bioactive ingredients in large scale within 1 h. Four major breynins were purified, and their structures were elucidated by analysis of MS and NMR data. Breynins can significantly prevent the rats' arthritis deterioration, with inhibition ratio 50% at dose 0.2 mg kg−1, comparable with that of indomethacin at dose 2 mg kg−1.The breynins have strong anti-arthritis activities, which is responsible to the anti-inflammatory effects of B. fruticosa. However, breynins are also toxic components of B. fruticosa.