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The identification and isolation of anti-inflammatory ingredients of ethno medicine Breynia fruticosa

Author:
He, Xiao-Li, Lv, Jun-Jiang, Wang, Xu, Zhang, Qing, Zhang, Bei, Cao, Kun, Liu, Lu-Lu, Xu, Yi
Source:
Journal of ethnopharmacology 2019 v.239 pp. 111894
ISSN:
0378-8741
Subject:
Breynia, adjuvants, animal models, anti-inflammatory activity, arthritis, eczema, gastroenteritis, high performance liquid chromatography, indomethacin, ingredients, mass spectrometry, nuclear magnetic resonance spectroscopy, permeability, pharyngitis, rats, resins, sesquiterpenoids, sulfur, tannins, toxicity, traditional medicine, China
Abstract:
Breynia fruticosa is a folk medicine in China, traditionally used to treat gastroenteritis, sore throat, eczema and arthritis. However, the bioactive ingredients are unknown.To identify and isolate the anti-inflammatory ingredients of B. fruticosa.B. fruticosa extracts were fractioned by Amberchrom CG161M and Toyopearl HW40C resins. Acetic acid-induced capillary permeability mice model was used to evaluate the anti-inflammation activities of fractions. The anti-inflammatory ingredients were identified by high performance liquid chromatography/mass spectroscopy (HPLC-MS). On-line two dimensional liquid chromatography system was constructed to remove the tannins and enrich the breynins. The breynins were purified by preparative HPLC and evaluated for their anti-arthritis activities using complete Freund's adjuvant (CFA) induced arthritis rats model.The anti-inflammatory ingredients of B. fruticosa are sulfur containing sesquiterpenoids (breynins). The on-line two dimensional preparative liquid chromatography system can effectively remove the tannins and enrich the bioactive ingredients in large scale within 1 h. Four major breynins were purified, and their structures were elucidated by analysis of MS and NMR data. Breynins can significantly prevent the rats' arthritis deterioration, with inhibition ratio 50% at dose 0.2 mg kg−1, comparable with that of indomethacin at dose 2 mg kg−1.The breynins have strong anti-arthritis activities, which is responsible to the anti-inflammatory effects of B. fruticosa. However, breynins are also toxic components of B. fruticosa.
Agid:
6380184