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Comparative anti-inflammatory effects of pentoxifylline, polymyxin B, tyloxapol and flunixin on acute phase proteins, antioxidants and cardiovascular biomarkers during inflammatory responses in dromedary calves

Author:
Samimi, Amir Saeed, Karimiafshar, Marzieh, Samimi, Kamyab
Source:
Small ruminant research 2019 v.175 pp. 78-82
ISSN:
0921-4488
Subject:
Camelus dromedarius, Escherichia coli, acute phase proteins, amyloid, anti-inflammatory activity, antioxidants, biomarkers, blood serum, calves, flunixin, glutathione peroxidase, haptoglobins, heart rate, hematocrit, inflammation, intravenous injection, leukocytes, lipopolysaccharides, males, pentoxifylline, phenol, polymyxin B, respiratory rate, serotypes, superoxide dismutase, temperature, troponin I
Abstract:
The present study aims to 1- assessment of acute phase proteins, antioxidants and cardiovascular biomarkers during inflammatory response which was induced by Escherichia coli (E. coli) lipopolysaccharide (LPS) in dromedary calves and 2- comparatively evaluate the anti-inflammatory effects of intravenous (IV) pentoxifylline, polymyxin B, tyloxapol and flunixin thereafter. A total of thirty male dromedary calves with the age of 20 ± 2 weeks and weight of 100 ± 5 kg. The calves were assigned randomly to five experimental groups (4 treatment and 1 positive control). Phenol extracted LPS at 0.1 μg kg−1 from E.coli serotype O55:B5 was used to induce inflammation. Six hours after induction of inflammatory responses, calves in groups Pntx, Plmx, Tlxp and Flnx IV received pentoxifylline (60 mg kg-1), polymyxin B (6000 U kg-1), tyloxapol (400 mg kg-1) and flunixin (2.2 mg kg-1), respectively. Calves in the positive control group were assigned to receive LPS without any anti-inflammatory drugs. Our results showed that values of heart rate (HR), respiratory rate (RR), rectal temperature (RT), packed cell volume (PCV), white blood cell (WBC), haptoglobin (HP), serum amyloid A (SAA), cardiac troponin I (cTnI) and hemocysteine (Hcy) were increased and glutathione peroxidase (GPx) and superoxide dismutase (SOD) were decreased during inflammatory responses in dromedary calves. According to our results, RR and SAA after 12 h, HR, RT, cTnI, Hcy and PCV after 18 h and GPx after 24 h were not significantly different from baseline within the Flnx group. These effects were not observed in other treatment groups. Base on our results there was no superiority in anti-inflammatory effects among pentoxifylline, polymyxin B and tyloxapol during inflammatory responses in dromedary calves. Flunixin meglumine (at 2.2 mg kg-1) can be considered useful for inflammatory conditions in dromedary calves.
Agid:
6380282