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Pulp improvement of oil palm empty fruit bunches associated to solid-state biopulping and biobleaching with xylanase and lignin peroxidase cocktail produced by Aspergillus sp. LPB-5

Orozco Colonia, Brigitte Sthepani, Lorenci Woiciechowski, Adenise, Malanski, Rodrigo, Junior Letti, Luiz Alberto, Soccol, Carlos Ricardo
Bioresource technology 2019 v.285 pp. 121361
Aspergillus, Elaeis guineensis, biobleaching, biopulping, cellulose, digestibility, endo-1,4-beta-glucanase, enzyme activity, feedstocks, fruits, fungi, hemicellulose, lignin, lignin peroxidase, lignocellulose, pretreatment, pulp, xylanases
Oil palm empty fruit bunches is a lignocellulosic feedstock with biotechnological potential and thousands of tons are generated in the world each year. Filamentous fungi producing xylanases and ligninases in biopulping to obtain cellulose is a pulp improvement alternative. The enzymatic cocktail was produced in solid-state biopulping by Aspergillus sp. LPB-5 with 54.32 U/g xylanase, 13.41 U/g lignin peroxidase and low cellulase activity. Biological, thermal and chemical pretreatments were compared and enzymatic biobleaching was applied to pretreated pulps. Biopulping and biobleaching combination had 36.80% lignin loss, 26.27% hemicellulose reduction, 74.36% pulp yield with 36.56% digestibility. Alkaline and biobleaching combination removed 81.97% hemicellulose and 93.89% lignin with 73.59% digestibility. Enzymatic biobleaching increased the pulp digestibility in all pretreatments. Finally, the development of a bio-pretreatment to remove hemicellulose and alter the lignin-carbohydrate complex interface presented a soft process with great eco-friendly potential, where mild pre-treatments would reduce the use of aggressive agents.