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Spotted knifejaw (Oplegnathus punctatus) MyD88: Intracellular localization, signal transduction function and immune responses to bacterial infection

Author:
Liu, Xiaobing, Li, Xuemei, Du, Xinxin, Sun, Minmin, Wang, Xuangang, Li, Wensheng, Zhai, Jieming, Liu, Jinxiang, Yu, Haiyang, Zhang, Quanqi
Source:
Fish & shellfish immunology 2019 v.89 pp. 719-726
ISSN:
1050-4648
Subject:
Oplegnathus, Toll-like receptors, Vibrio anguillarum, apoptosis, bacterial infections, cytoplasm, fish, gills, immune response, innate immunity, interleukin-1, kidneys, liver, mammals, pathogens, quantitative polymerase chain reaction, signal transduction, spleen, tissues, transcription factor NF-kappa B, China
Abstract:
Myeloid differentiation factor 88 (MyD88) links members of the toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) superfamily to the downstream activation of NF-κB as a “bridge” molecular in response to exogenous pathogen, but the function in spotted knifejaw (Oplegnathus. punctatus), a commercial fish in China, is still unknown. We present a functional analysis of spotted knifejaw MyD88 (OppMyD88) with a typical death domain (DD) at the N-terminus and a conservative Toll/IL-1R (TIR) domain at the C-terminus and suggest that MyD88 is important for the activation of TLR-mediated NF-κB with the synergy between domains. Subcellular localization showed that OppMyD88 was distributed in the cytoplasm in a condensed form. Tissues expression profiling analysis showed that OppMyD88 ubiquitously expressed in all tested tissues with the highest expression in the liver, as determined by real-time PCR. The expression of OppMyD88 significantly upregulated in the liver, spleen, kidney and gills within 120 h post Vibrio anguillarum infection. Moreover, we further confirmed that over-expressed OppMyD88 could also induce apoptosis. These results indicate that OppMyD88 might possess important roles in defense against microbial infection and other biological processes in spotted knifejaw similar to those in mammals, which will deepen our understandings in innate immunity of spotted knifejaw.
Agid:
6380758