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Infection of Spathoglottis plicata (Orchidaceae) seeds by mycorrhizal fungus
- Tan, T. K., Loon, W. S., Khor, E., Loh, C. S.
- Plant cell reports 1998 v.18 no.1-2 pp. 14-19
- Rhizoctonia, Spathoglottis, alginates, chitosan, coculture, encapsulation, gelatin, mycorrhizal fungi, polymers, seeds, sucrose, symbiosis
- Spathoglottis plicata seeds were encapsulated in 4-mm-diameter capsules of alginate-chitosan or alginate-gelatin and infected with the mycorrhizal fungus Rhizoctonia AM9. The encapsulated seeds were placed directly on Rhizoctonia culture. About 66% of the seeds encapsulated in sucrose-free chitosan-alginate established a symbiotic relationship with the mycorrhizal fungus after co-culturing for 2 weeks. The highest percentage of infection observed was about 84%. Addition of sucrose or using gelatin-alginate for encapsulation reduced the percentage of infection by about half. The growth of Rhizoctonia AM9 in sucrose-free alginate, chitosan and gelatin was found to be minimal. The advantages of germinating orchid seeds, encapsulated in sucrose-free polymers, through mycorrhizal infection is discussed.