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Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications
- Leemhuis, Hans, Kelly, Ronan M., Dijkhuizen, Lubbert
- Applied microbiology and biotechnology 2010 v.85 no.4 pp. 823-835
- bakery industry, catalysts, cyclodextrins, cyclomaltodextrin glucanotransferase, engineering, engineers, enzyme activity, oligosaccharides, protein engineering, screening, starch
- Cyclodextrin glucanotransferases (CGTases) are industrially important enzymes that produce cyclic α-(1,4)-linked oligosaccharides (cyclodextrins) from starch. Cyclodextrin glucanotransferases are also applied as catalysts in the synthesis of glycosylated molecules and can act as antistaling agents in the baking industry. To improve the performance of CGTases in these various applications, protein engineers are screening for CGTase variants with higher product yields, improved CD size specificity, etc. In this review, we focus on the strategies employed in obtaining CGTases with new or enhanced enzymatic capabilities by searching for new enzymes and improving existing enzymatic activities via protein engineering.