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A multipurpose biochip for food pathogen detection

Author:
Primiceri, Elisabetta, Chiriacò, Maria Serena, de Feo, Francesco, Santovito, Elisa, Fusco, Vincenzina, Maruccio, Giuseppe
Source:
Analytical methods 2016 v.8 no.15 pp. 3055-3060
ISSN:
1759-9679
Subject:
Listeria monocytogenes, Staphylococcus aureus, analytical methods, bacteria, biosecurity, culture media, detection limit, diagnostic techniques, food pathogens, foods, ingestion, microbial detection, point-of-care systems, public health, toxins, virulent strains
Abstract:
Foodborne illnesses caused by the ingestion of foods contaminated with pathogens and/or their toxins are still one of the major public health threats worldwide. Disposable devices, allowing the on-site, early and multiplexed quantitative detection of pathogenic bacteria are therefore highly sought. Herein, we report biochips that are able to quantitatively detect two of the most common food-associated pathogens, namely Listeria monocytogenes and Staphylococcus aureus from the suspensions of bacteria stationary-phase broth culture. With a detection limit as low as 5.00 CFU ml⁻¹ for L. monocytogenes and 1.26 CFU ml⁻¹ for S. aureus, our platform may be a promising point-of-care device not only for clinical and food diagnostics but also for biosecurity purposes.
Agid:
6383647