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Silver nanoparticle@DNA tetrahedron-based colorimetric detection of HIV-related DNA with cascade strand displacement amplification

Author:
Ma, Xiaoyi, Miao, Peng
Source:
Journal of materials chemistry B 2019 v.7 no.16 pp. 2608-2612
ISSN:
2050-7518
Subject:
DNA, biosensors, blood serum, colorimetry, detection limit, nanosilver, polymerization
Abstract:
DNA tetrahedron-modified silver nanoparticles (AgNPs) were achieved via amino-silver chemistry for the first time and were applied as a colorimetric biosensor for detecting HIV-related DNA. Target DNA initiated strand displacement polymerization and nicking endonuclease-aided cycles were involved to link DNA tetrahedron-modified AgNPs, reporting colorimetric responses. This developed method showed excellent specificity and sensitivity. A wide linear range from 1 to 15 000 nM was achieved with a limit of detection of 0.84 nM. Moreover, it was successfully applied to determine DNA in blood serum samples.
Agid:
6385502