PubAg

Main content area

Structural characterization of EasH (Aspergillus japonicus) – an oxidase involved in cycloclavine biosynthesis

Author:
Jakubczyk, Dorota, Caputi, Lorenzo, Stevenson, Clare E. M., Lawson, David M., O'Connor, Sarah E.
Source:
Chemical communications 2016 v.52 no.99 pp. 14306-14309
ISSN:
1364-548X
Subject:
Aspergillus japonicus, biosynthesis, chemical reactions, ergot alkaloids, fungi, oxidoreductases
Abstract:
Aj_EasH is a non-heme iron- and α-keto-glutarate-dependent oxidase that is responsible for an unusual cyclopropyl ring formation in the biosynthesis of the fungal ergot alkaloid cycloclavine. The three dimensional structure of Aj_EasH (2.2 Å resolution) reported here provides insight into the mechanism of this unusual and complex reaction.
Agid:
6390273