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A combination strategy using two novel cerium-based nanocomposite affinity probes for the selective enrichment of mono- and multi-phosphopeptides in mass spectrometric analysis
- Long, Xing-yu, Zhang, Zi-jin, Li, Jia-yuan, Sheng, Dong, Lian, Hong-zhen
- Chemical communications 2017 v.53 no.33 pp. 4620-4623
- cerium, chemical reactions, matrix-assisted laser desorption-ionization mass spectrometry, nanocomposites, phosphopeptides, spectral analysis
- This work presents a sequential enrichment protocol, based on two self-designed cerium-based nanocomposite affinity probes, which not only can effectively separate phosphopeptides from non-phosphopeptides but can also selectively differentiate mono- and multi-phosphopeptides for direct matrix-assisted laser desorption/ionization time-of-flight mass spectrometric (MALDI-TOF MS) analysis.