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Portable detection of colorectal cancer SW620 cells by using a personal glucose meter

Zhou, Jun, Duan, Lunxi, Huang, Jiangsheng, Zuo, Zhongkun, Tang, Tenglong, Cao, Ding, Ye, Fei, Zhou, Shiwei, Zhou, Yueying, Zhang, Leiyi
Analytical biochemistry 2019 v.577 pp. 110-116
ambient temperature, beta-fructofuranosidase, blood serum, colorectal neoplasms, detection limit, glucose, graphene oxide, humans, magnetic materials, neoplasm cells, oligonucleotides, point-of-care systems
It is of great value to develop general, low-cost and even household methods for colorectal cancer detection. Here, a portable detection strategy based on a personal glucose meter (PGM) was designed for meeting this purpose. In this strategy, the anti-EpCAM coated magnet beads (MBs) were used as capture probes for enriching cancer cells and the aptamer modified and invertase loaded graphene oxides (GO) were used as report probes for producing glucose signal. This method is sensitive with detection limit as low as 560 cells, and demonstrates excellent detection specificity. Meanwhile, we succeeded in the specific detection of target cells in 20% human serum samples, indicating this method has great prospect in clinical diagnosis. Moreover, this method presents favourable universality for detecting different colorectal cancer cells by just using different recognition aptamers. Importantly, this method can be implemented for the target cell detection at room temperature without any expensive and large-scale instruments but a portable PGM. Therefore, this portable detection method possesses great potential in point-of-care detection of colorectal cancer cells.