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Double layer co-encapsulation of probiotics and prebiotics by electro-hydrodynamic atomization

Author:
Zaeim, Davood, Sarabi-Jamab, Mahboobe, Ghorani, Behrouz, Kadkhodaee, Rassoul
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2019 v.110 pp. 102-109
ISSN:
0023-6438
Subject:
Bifidobacterium animalis subsp. lactis, Lactobacillus plantarum, ambient temperature, atomization, bacteria, calcium alginate, chitosan, coatings, colon, encapsulation, foods, inulin, molecular weight, prebiotics, probiotics, protective effect, resistant starch, storage temperature, viability
Abstract:
Lactobacillus plantarum and Bifidobacterium lactis were co-encapsulated separately with either inulin or resistant starch in Ca-alginate/chitosan microcapsules by electro-hydrodynamic atomization (EHDA). Encapsulation yield of core materials and their release profile were studied. Protective role of polysaccharide matrix on bacteria under gastrointestinal (GI) conditions and storage at 25, 4 or −18 °C were investigated. Encapsulation yield was calculated to be 98, 79 and 99 percent for bacteria, inulin and resistant starch, respectively. Ten percent of inulin was expelled from the Ca-alginate network into water after 24 h; however, no resistant starch was released. Chitosan coating reduced the release of inulin by up to 5 percent. Survival studies showed 5.90 ± 0.3 log CFU/g of Lb. plantarum and 7.19 ± 0.15 log CFU/g of B. lactis survived GI conditions in starch-containing microcapsules. Viability loss of Lb. plantarum was successfully restricted during storage at room temperature by encapsulation in Inulin-containing microcapsules as 6.33 ± 0.21 log CFU/g survived after 90 days storage. The findings revealed EHDA can be employed for co-encapsulation of probiotics and high molecular weight prebiotics in micron-sized capsules which could open up a new perspective for targeted delivery of live cells to the colon through foods.
Agid:
6392660