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A mitochondria-target probe for OCl− “naked eye” detection and its imaging in living cell

Author:
Zhong, Xiuli, Zhou, Lin, Jin, Can, Wang, Bingxiang, Jiang, Yuliang, Shen, Jian
Source:
Talanta 2019 v.202 pp. 369-374
ISSN:
0039-9140
Subject:
chemical bonding, color, detection limit, fluorescence, fluorescent dyes, homeostasis, human health, image analysis, toxicity
Abstract:
Hypochloric acid (HOCl) plays vital roles in cell signaling and homeostasis which involved in many related diseases. Therefore, the accurate detected level of OCl− for discussing the complex contributions of OCl− to human health is of great significance. In this study, a novel mitochondrion-targeting fluorescent probe DMI bearing a carbon-carbon double bond as an OCl−-responsive site has been developed. Probe DMI exhibited specific fluorescence response toward OCl− with fast response (within 4 s) and high sensitivity (detection limit is 0.05 μM), where the obvious color changes could be observed by the naked eye. More importantly, DMI could be applied in the bioimaging of OCl− in living A549 cells successfully benefited from its good sensing properties and low toxicity. Fluorescence co-localization test was further carried out and confirmed mitochondria-targetable ability of DMI which can be used for investigating physiological function of HOCl at organelle levels.
Agid:
6392817