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Pharmacokinetics and residue elimination of cynatratoside-C in the grass carp Ctenopharyngodon idellus following a single intraperitoneal injection

Author:
Fu, Yao-Wu, Zhang, Hui-Jie, Zhang, Jia-Ying, Zhang, Qi-Zhong, Li, Qing-Guo
Source:
Aquaculture 2019 v.508 pp. 186-191
ISSN:
0044-8486
Subject:
Ctenopharyngodon idella, Ichthyophthirius multifiliis, Vincetoxicum atratum, absorption, blood serum, body weight, fish, half life, high performance liquid chromatography, intraperitoneal injection, kidneys, liver, models, muscles, pharmacokinetics, tandem mass spectrometry, water temperature
Abstract:
Cynatratoside-C, isolated from Cynanchum atratum, is a promising alternative agent for the control of the ecoparasite Ichthyophthirius multifiliis. However, the pharmacokinetics and residue elimination of cynatratoside-C in fish have yet to be reported. Here, we aimed to investigate the pharmacokinetics and residue elimination of cynatratoside-C in grass carp following a single intraperitoneal (i.p.) dose of 2 mg/kg body weight at a water temperature of 23.0 ± 0.3 °C. The concentrations of cynatratoside-C in the serum, liver, kidney, and muscle were determined using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). We found that the pharmacokinetics of cynatratoside-C in the grass carp was described by a two-compartment model. The absorption half-life (t₁/₂Kα), distribution half-life (t₁/₂α), and elimination half-life (t₁/₂ᵦ) of cynatratoside-C in the serum were 0.28 h, 1.45 h, and 69.32 h, respectively. The maximum cynatratoside-C concentration (Cₘₐₓ) in the serum was 123.81 μg/L, with a time to peak concentration (Tₘₐₓ) of 0.5 h. The post-injection residue concentration of cynatratoside-C (during the elimination period) was highest in the liver and lowest in the muscle. The concentration of cynatratoside-C in the muscle fell below the limit of quantification (1.24 ng/g) 15 days post-administration. Thus, for grass carp, we recommend a withdrawal period of 15 days following a single i.p. dose of 2 mg cynatratoside-C/kg body weight at a water temperature of 23.0 ± 0.3 °C.
Agid:
6392911