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Decoding and analysis of organelle genomes of Indian tea (Camellia assamica) for phylogenetic confirmation

Rawal, Hukam C., Kumar, P. Mohan, Bera, Biswajit, Singh, Nagendra Kumar, Mondal, Tapan Kumar
Genomics 2019
Camellia sinensis var. assamica, Caryophyllales, chloroplast genome, chloroplasts, databases, genes, mitochondria, mitochondrial genome, nucleotide sequences, phylogeny, ribosomal RNA, tea, transfer RNA
The NCBI database has >15 chloroplast (cp) genome sequences available for different Camellia species but none for C. assamica. There is no report of any mitochondrial (mt) genome in the Camellia genus or Theaceae family. With the strong believes that these organelle genomes can play a great tool for taxonomic and phylogenetic analysis, we successfully assembled and analyzed cp and mt genome of C. assamica. We assembled the complete mt genome of C. assamica in a single circular contig of 707,441 bp length comprising of a total of 66 annotated genes, including 35 protein-coding genes, 29 tRNAs and two rRNAs. The first ever cp genome of C. assamica resulted in a circular contig of 157,353 bp length with a typical quadripartite structure. Phylogenetic analysis based on these organelle genomes showed that C. assamica was closely related to C. sinensis and C. leptophylla. It also supports Caryophyllales as Superasterids.