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l- histidine and l-carnosine accelerate wound healing via regulation of corticosterone and PI3K/Akt phosphorylation in d-galactose-induced aging models in vitro and in vivo

Kim, Yerin, Kim, Eunju, Kim, Yuri
Journal of functional foods 2019 v.58 pp. 227-237
cadherins, carnosine, collagen, corticosterone, elderly, galactose, histidine, keratinocytes, mice, models, mortality, phosphorylation, tissue repair
Impaired skin wound healing in the elderly can lead to medical issues and increased mortality. Although l-histidine and l-carnosine are potent anti-aging amino acids, the wound healing effects of these amino acids in aging remain to be elucidated. Here, we investigated the regenerative potential of l-histidine and l-carnosine in in vitro and in vivo aging models. l-histidine (1 mM), l-carnosine (10 mM), or a combination improved proliferation, migration, senescence, and epithelial-mesenchymal transition (EMT) in d-galactose-induced aged keratinocytes. An in vivo mouse aging model was established with injection of d-galactose (s.c. 500 mg/kg) daily for eight weeks. Supplementation with l-histidine (2 g/L), l-carnosine (2 g/L), or a combination improved collagen and wound healing with EMT markers, E-cadherin, N-cadherin, and MMP-2. These effects were concomitant with reduced circulating levels of corticosterone and increased PI3K/Akt phosphorylation. These results suggest that l-histidine and l-carnosine have the potential to facilitate wound healing in aging skin.