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Detection of N⁶-methyladenosine based on the methyl-sensitivity of MazF RNA endonuclease

Author:
Imanishi, Miki, Tsuji, Shogo, Suda, Akiyo, Futaki, Shiroh
Source:
Chemical communications 2017 v.53 no.96 pp. 12930-12933
ISSN:
1364-548X
Subject:
Escherichia coli, RNA, chemical compounds, chemical reactions, enzyme activity, methyltransferases, screening
Abstract:
We found that Escherichia coli MazF toxin, an ACA-sequence-specific endoribonuclease, was sensitive to N⁶-methyladenosine (m6A), representing the first m6A-sensitive RNA cleavage enzyme. The methyl-sensitivity of MazF allowed simple analyses of both m6A demethylase and methyltransferase activity. Furthermore, the approach could be used for inhibitor screening.
Agid:
6396972