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Inhibition of cell proliferation and triggering of apoptosis by agrimonolide through MAP kinase (ERK and p38) pathways in human gastric cancer AGS cells

Author:
TengAuthors contributed equally to the work., Hui, Huang, Qun, Chen, Lei
Source:
Food & function 2016 v.7 no.11 pp. 4605-4613
ISSN:
2042-650X
Subject:
B-lymphocytes, apoptosis, caspase-3, cell proliferation, dose response, enzyme inhibitors, flow cytometry, humans, mitogen-activated protein kinase, signal transduction, stomach neoplasms
Abstract:
Data from the present study showed that agrimonolide exhibited a high anti-proliferation effect against human gastric cancer AGS cells. Flow cytometric analysis revealed that the number of total apoptotic cells increased after the treatment with the agrimonolide in a dose-dependent manner. In addition, it was found that agrimonolide-induced cell apoptosis was associated with the increase in the (Bcl-2 Associated X Protein, BAX)/(B-cell lymphoma-2, Bcl-2) ratio and the activation of cleaved caspase-3. MAPK (p38, c-Jun N-terminal kinase, and ERK1/2) signaling pathways were involved in agrimonolide-induced apoptosis. Cells were exposed to 40 μM of agrimonolide and the level of phospho-ERK/ERK protein was increased to 7.0-fold as compared to the control, and the expression of phospho-p38 protein showed a significant 6.2-fold increase after 24 h incubation, as compared to the control. The employment of protein kinase inhibitors of PD98059 and SB203580, showed the block effects of agrimonolide on the activation of caspase-3 and apoptosis.
Agid:
6398551