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New tools for reconstruction and heterologous expression of natural product biosynthetic gene clusters
- Luo, Yunzi, Enghiad, Behnam, Zhao, Huimin
- Natural product reports 2016 v.33 no.2 pp. 174-182
- DNA assembly, biosynthesis, genetically modified organisms, heterologous gene expression, humans, metabolic engineering, multigene family, synthetic biology, therapeutics
- Covering: 2010 to 2015 Natural product scaffolds remain a major source and inspiration for human therapeutics. However, generation of a natural product in the post-genomic era often requires reconstruction of the corresponding biosynthetic gene cluster in a heterologous host. In the burgeoning fields of synthetic biology and metabolic engineering, a significant amount of efforts has been devoted to develop DNA assembly techniques with higher efficiency, fidelity, and modularity, and heterologous expression systems with higher productivity and yield. Here we describe recent advances in DNA assembly and host engineering and highlight their applications in natural product discovery and engineering.