PubAg

Main content area

Metal ion-immobilized magnetic nanoparticles for global enrichment and identification of phosphopeptides by mass spectrometry

Author:
Zhai, Rui, Tian, Fang, Xue, Ruiqing, Jiao, Fenglong, Hao, Feiran, Zhang, Yangjun, Qian, Xiaohong
Source:
RSC advances 2015 v.6 no.2 pp. 1670-1677
ISSN:
2046-2069
Subject:
adsorption, alpha-casein, binding capacity, holmium, human cell lines, iron, magnetism, mass spectrometry, metal ions, models, nanoparticles, phosphopeptides, phosphoproteins, phosphoproteome, porphyrins, terbium, thulium, titanium, titanium dioxide
Abstract:
Phosphopeptide enrichment is critical for the deep analysis of the phosphoproteome, and many strategies have been developed to improve the coverage of identified phosphopeptides. However, the isolation of multiple phosphorylated peptides is still limited due to their distinct affinity capability to various materials. For example, titanium dioxide is preferred to isolate mono-phosphopeptides owing to its very strong binding of multiple phosphorylated peptides. Here, a new set of different metal ion (Ti⁴⁺, Zr⁴⁺, Fe³⁺, Tb³⁺, Tm³⁺, Ho³⁺)-immobilized magnetic nanoparticles, Fe₃O₄@TCPP-DOTA-Ms, were prepared, which possess the advantages of a stable metal complex, a large binding capacity and easy manual operation by introducing TCPP (tetrakis(4-carboxylphenyl) porphyrin), DOTA (1,4,7,10-tetraazacyclododecane N,N′,N′′,N′′′-tetraacetic acid) and various metal ions onto the magnetic nanoparticles. For the model protein α-casein tryptic digest, 15 phosphopeptides were identified with Fe₃O₄@TCPP-DOTA-Tb or Ti, and 46.67% of the enriched phosphopeptides were multiple phosphorylated peptides, indicating that the metal ion Tb³⁺ and Ti⁴⁺-immobilized materials have excellent enrichment efficiency and stronger adsorption for multiple phosphorylated peptides than other metal ion immobilized magnetic nanoparticles. Even in the tryptic digest of α-casein and BSA (1 : 50), 14–15 phosphopeptides were easily detected with Fe₃O₄@TCPP-DOTA-Tb/Ti, revealing that the novel materials possess strong selectivity for phosphopeptide enrichment. Additionally, Fe₃O₄@TCPP-DOTA-Tb/Ti was utilized to capture phosphopeptides in the tryptic digest of an extract of HeLa cells. In total, 13 450 phosphopeptides corresponding to 2965 phosphoproteins were identified in mass spectrometric analyses. The specificity for phosphopeptide enrichment was as high as 94%. More than half of the identified unique phosphopeptides were multiple phosphorylated peptides, which was much higher than that identified by the DHB/TiO₂ (13.39%) method, making these materials a good choice for highly selective and global phosphopeptide enrichment in phosphoproteome analysis.
Agid:
6400994