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An agile, simplified and sonication mediated one-pot aqueous extraction and antibacterial assessment of predominant Korean mushrooms

Shang, Xiaomin, Muthu, Manikandan, Keum, Young Soo, Chun, Sechul, Gopal, Judy
RSC advances 2016 v.6 no.15 pp. 12143-12157
Pseudomonas aeruginosa, Streptococcus mutans, antibacterial properties, bioactive compounds, gas chromatography-mass spectrometry, mushrooms, palmitic acid, pathogens, solvents, sonication, stearic acid
Solvent-based extractions have always held the upper hand when it comes to mushrooms. Assimilating the fact that mushrooms are a part of culinary components cooked in water and not in solvents; solvent-based extraction becomes a priority. Effective water based extraction stretches 24 h, leaving space for prospective improvising through analytical interference. We have demonstrated the effective downsizing of the extraction time from 24 h to 2 min via sonication based extraction strategies. A water bath-based method could achieve effective extraction at 30 min, whereas further enhancement was seen through the use of a probe sonication approach to 2 min. The extraction efficiency was tested based on the antibacterial activity of mushroom extracts against two pathogens, Streptococcus mutans and Pseudomonas aeruginosa. The systematic optimization of the sonication approach and a comparison of their effectiveness versus conventional approaches are demonstrated. The bioactive components in the extracts obtained via the different extractions have been characterized using biochemical characterization as well as GC-MS analysis. The enhanced extraction and potent role of butanoic acid, hexadecanoic acid, octadecanoic acid and 1,2-benzenedicarboxylic acid were confirmed to be behind the success behind the sonication mediated extraction.