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BMSCs-laden gelatin/sodium alginate/carboxymethyl chitosan hydrogel for 3D bioprinting
- Huang, Jie, Fu, Han, Wang, Zhiying, Meng, Qingyuan, Liu, Sumei, Wang, Heran, Zheng, Xiongfei, Dai, Jianwu, Zhang, Zhijun
- RSC advances 2016 v.6 no.110 pp. 108423-108430
- antibacterial properties, bioprinting, cell viability, chitosan, gelatin, hydrogels, mechanical properties, medicine, mesenchymal stromal cells, mixing, sodium alginate, stem cells, tissue engineering, water content
- Three-dimensional (3D) bioprinting technology offers the possibility to deliver, in a defined and organized manner, scaffolding materials and living cells, and therefore holds much promise for tissue engineering and regenerative medicine. In this work, we explored the possibility of gelatin/sodium alginate/carboxymethyl chitosan (Gel/SA/CMCS) hydrogel combining with bone mesenchymal stem cells (BMSCs) for 3D bioprinting. Compared to the commonly used 3D bioprinting materials such as gelatin/sodium alginate (Gel/SA) hydrogel, the Gel/SA/CMCS hydrogel we developed shows excellent equilibrium water content, good mechanical properties, antibacterial activity, and a slow degradation rate. With this kind of material, we generated a scaffold with homogenous cell distribution. Cell viability after 3D printing showed that over 85% of the printed cells were viable at 0 and 2 days of culturing. Our work demonstrates the feasibility of mixing Gel/SA/CMCS hydrogel with stem cells for 3D bioprinting.